Demonstration of feline infectious peritonitis virus in conjunctival epithelial cells from cats
Identifieur interne : 001C47 ( Main/Exploration ); précédent : 001C46; suivant : 001C48Demonstration of feline infectious peritonitis virus in conjunctival epithelial cells from cats
Auteurs : Kristina Hök [Suède]Source :
- APMIS [ 0903-4641 ] ; 1989-07.
English descriptors
- Teeft :
- Cell cultures, Cell line, Clinical practice, Conjunctival epithelial cells, Epithelial, Epithelial cells, Feline, Feline coronaviruses, Fipv, Fipv disease, Fipv infection, Immunofluorescence, Immunofluorescence studies, Immunohistopathological methods, Karolinska institutet, Leighton tubes, Local veterinarians, Membrana, Membrana nictitans, Mucous membranes, National board, Negative controls, Nictitans, Peritonitis, Peritonitis virus, Positive serum, Present investigation, Reliable method, Screen infection table, Serological, Serological test, Serological tests, Small anim, Smear, Sucrose solution, Tissue cultures, Vaginal smears, Viral, Viral suspension.
Abstract
A simple technique demonstrating the presence of feline infectious peritonitis virus (FIPV) in cats is described. Smears from the third eyelid (membrana nictitans) were dried on microscope slides, fixed in aetone, and stained using an indirect immunofluorescence method with rabbit‐anti‐FIPV serum. By this method FIPV‐antigen could be demonstrated in the cytoplasma of epithelial cells. The results obtained with this method (M3) were compared with serological and immunohistopathological methods for confirming FIPV‐infection in cats. The results obtained by the M3 test were up to 85% in concordance with these other tests. Furthermore, the M3 test was easier and quicker to perform and should be well suited for clinical practice.
Url:
DOI: 10.1111/j.1699-0463.1989.tb00483.x
Affiliations:
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Le document en format XML
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Smears from the third eyelid (membrana nictitans) were dried on microscope slides, fixed in aetone, and stained using an indirect immunofluorescence method with rabbit‐anti‐FIPV serum. By this method FIPV‐antigen could be demonstrated in the cytoplasma of epithelial cells. The results obtained with this method (M3) were compared with serological and immunohistopathological methods for confirming FIPV‐infection in cats. The results obtained by the M3 test were up to 85% in concordance with these other tests. Furthermore, the M3 test was easier and quicker to perform and should be well suited for clinical practice.</div></front></TEI><affiliations><list><country><li>Suède</li></country><region><li>Svealand</li></region><settlement><li>Stockholm</li></settlement></list><tree><country name="Suède"><region name="Svealand"><name sortKey="Hok, Kristina" sort="Hok, Kristina" uniqKey="Hok K" first="Kristina" last="Hök">Kristina Hök</name></region><name sortKey="Hok, Kristina" sort="Hok, Kristina" uniqKey="Hok K" first="Kristina" last="Hök">Kristina Hök</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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